An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples

Recovery of Organisms and Nucleic Acids from Complex Samples

167 The process of recognizing, identifying, and responding to a biological or chemical attack requires multiple stages of sample and information collection and processing. There are several methods for identifying target organisms and nucleic acids in a sample. These methods can be broadly divided into categories, the most commonly used of which are the polymerase chain reaction (PCR), immunol...

An instrument for automated purification of nucleic acids from contaminated forensic samples.

Forensic crime scene sample analysis, by its nature, often deals with samples in which there are low amounts of nucleic acids, on substrates that often lead to inhibition of subsequent enzymatic reactions such as PCR amplification for STR profiling. Common substrates include denim from blue jeans, which yields indigo dye as a PCR inhibitor, and soil, which yields humic substances as inhibitors....

A Microfluidic Device for Structural Studies of Nucleic Acids

We assembled a microfluidic device that enables highresolution structural studies of nucleic acids. The device contains a mineral matrix capable of rapidly generating hydroxyl radicals to cleave the solvent accessible backbone of nucleic acids. Protection from radical cleavage (footprinting) can identify protein binding sites or the presence of tertiary structure. Here we report the fabrication...

Circulating nucleic acids in plasma and serum: an overview.

The recent interest in nucleic acids in plasma and serum has opened up numerous new areas of investigation and new possibilities for molecular diagnosis. In oncology, tumor-derived genetic changes, epigenetic alterations, and viral nucleic acids have been found in the plasma/serum of cancer patients. These findings have important implications for the detection, monitoring, and prognostication o...

Human brucellosis: An overview